@article {117,
	title = {Exploring Translational Control of Maternal mRNAs in Zebrafish.},
	journal = {Methods Mol Biol},
	volume = {2218},
	year = {2021},
	month = {2021},
	pages = {367-380},
	abstract = {<p>The study of translational regulation requires reliable measurement of both mRNA levels and protein synthesis. Cytoplasmic polyadenylation is a prevalent mode of translational regulation during oogenesis and early embryogenesis. Here the length of the poly(A) tail of an mRNA is coupled to its translatability. We describe a protocol to identify translationally regulated genes and measure their translation rate in the early zebrafish embryo using genome-wide polysome profiling. This protocol relies on the isolation of mRNA by means of an rRNA depletion strategy, which avoids capture bias due to short poly(A) tail that can occur when using conventional oligo(dT)-based methods. We also present a simple PCR-based method to measure the poly(A) tail length of selected mRNAs.</p>},
	keywords = {Animals, Cytoplasm, Embryo, Nonmammalian, Embryonic Development, Oocytes, Oogenesis, Poly A, Polyadenylation, Protein Biosynthesis, RNA, Messenger, Stored, Zebrafish},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-0970-5_29},
	author = {Winata, Cecilia Lanny and {\L}api{\'n}ski, Maciej and Ismail, Hisyam and Mathavan, Sinnakaruppan and Sampath, Prabha}
}
@article {71,
	title = {Expression of components of Wnt and Hedgehog pathways in different tissue layers during lung development in Xenopus laevis.},
	journal = {Gene Expr Patterns},
	volume = {10},
	year = {2010},
	month = {2010 Oct-Dec},
	pages = {338-44},
	abstract = {<p>Although Wnt and Hedgehog (Hh) signaling pathways play important roles in mouse lung development, these have not been explored in the development of Xenopus lung. This may be due to the lack of specific molecular markers for different layers of tissue in Xenopus lung and/or insufficient knowledge on expression patterns of Wnt and Hh signaling components in Xenopus lung. In this study, we first described the early morphogenesis of Xenopus laevis lung by using surfactant protein C (sftpc) as a marker of lung epithelium and compared it with the expression patterns of several genes of Wnt and Hh pathways in Xenopus lungs. Our data showed that wnt7b was expressed in the entire lung epithelium from stage 37 to stage 45, while two other Wnt signaling components, wnt5a and wif1 (wnt inhibitory factor 1), were expressed in the mesenchyme layer of the entire lungs through stages 39-41. We also found that sonic hedgehog (shh) was expressed at stage 41 only in the anterior, but not in the posterior part of the lungs. These results show the expression of wnt5a, wnt7b, wif1 and shh in different layers of tissue of Xenopus lungs at early developmental stages, which implies different roles of these genes in the early development of Xenopus lungs. Our study for the first time defined specific molecular markers for description of early lung development in Xenopus, as well as provided information about expression of components of Wnt and Hh pathways in early Xenopus lungs, which should be useful for future functional studies.</p>},
	keywords = {Animals, Epithelium, Gene Expression Regulation, Developmental, Genetic Markers, Hedgehog Proteins, In Situ Hybridization, Lung, Mesoderm, Morphogenesis, Polymerase Chain Reaction, Pulmonary Surfactants, Signal Transduction, Wnt Proteins, Xenopus Proteins, Xenopus laevis},
	issn = {1872-7298},
	doi = {10.1016/j.gep.2010.07.005},
	author = {Yin, Ao and Winata, Cecilia L and Korzh, Svitlana and Korzh, Vladimir and Gong, Zhiyuan}
}
