EGFP expression in the notochord, skin, pectoral fin and branchial arches.

Insertion ET17-1

Genomic location: Chromosome 19

The insertion is in intron of cratb (carnitine acetyltransferase b)

DNA Sequence

Insertion ET17-2

Genomic location: Chromosome 2

The insertion is 7.8 kb upstream of im: 7138239

DNA Sequence

5’

3'

CATATTGTTATGCAATAAACTAATTCTTTTAGATTTCGTCTAAATGTAA

GTATTCCTACAAAACAAAGTTTTAAGTTACAAGTTTTAAGTGTGTACAA

GACATGGATAAAGCACCTTTTTCAGAGCTTAAAACTACTGTAAATTTAA

ATTAATTTAACAAGTTGCGTACCATGCTGGGAAAAATTAATACATTTTT

TATTACCTTTGTCTATTTTTACCTTTATTAAACATGTATTTTTAAGGCA

TATATTAACCAAACTTCTGAGTGTGATATGCGTGCCTGATTGAGAGAGA

TTAAGGAGACTGGGAAAGAGAGGGTATGACGCAGAACTTGGGTGCACCC

AGTCAACCATTGTAGGAGACAATATGGTACATATTACCATCTTGTGGTA

GAATTGCGACTGCATCATGACCGCCACCTAAAATACTTCAGG

GCTGCAGCTGATTCTGCCAACAGAGGAGGAGTAACAGTAAGTGTTTGT

TTCTGTTTCTCCGCCCTCCTCTGAATTTTTTCTTATTCAATCATTTCC

CAAATGATGTTTAACAGAGCGGGGAATTTTTCACTGTATTTCCTATAA

TTTTTTTTCTTCTGAAGAAAGTCTTATTTGCTTTATTTCGTTTTTAAC

TTTTTAAAAAATCATTTAAAGTCAATAGTATTATGTTGTATACTGTAC

GTGTTTAGGTTGTTACAGAACCCCAGAAGGGACGTAGTGGAGGAGAAA

AAAATTGGCTGGGTGAAAAAAAAAAAAGGGGGAAAAAAAAAAA

3’

ACAATATGTATGACAGATGTATCTCACAATACTAGCAACACAGTCAATG

CAGTGTAATTTCACCAAAAGGTTGTAGCAGTGGATTTGTGACAGAAAAA

AAAGGTTTCCTTACAATAGCATTAATGCAAAGACATCAGGATATAAGTC

AATTCTATATTATCTTTGTGAACGGAAATTCTCAAAAATGTTTGAGTAG

ACTGTGCTGAATGACACTATAACATAAGTATTCTAACTACTTCAAAATG

TATACTAGTGTATAAAAGACAAAGATATTGAGCATGTATGTCGCTAAGA

ATGTCATTTACCAGTGTCTGAAATACAGATGGCGCATTGACCAGCCCAA

GAAAAGAACCCAGTAATCAAAGTAAACAAAGAGAGCATTAATGATTTTC

CATTCATCATCCTCCCTGGATGTGCACAGAAATGATATGCATTATGGAG

ATCCAATTTGATAAAAACACTTGTTCCTCGTA