EGFP expression at the midbrain, hindbrain, branchial arches, retinal ganglial cells, mucous cells (3d) and corpuscles of Stannius (7d).

Genomic location: Chromosome 4

The insertion is in intron of acss3 (acyl-CoA synthetase short-chain family member 3)

DNA sequence

5’

3'

GTGTCTTTAGGCCAATCAAAAACAGCTAAGACGAACGCTTCCTGTGTGA

ACGCCCCTTTTGTGTAGTTTTCTCATTTCAGTCAGTAGCAAGTGGTAAA

CAAATAAATATTTACGTTTTTTTGTTTTAATTTAGTTCAGGTTTATGAT

GGCAGATTTTGTATGCTGTGGATTGACATGAGAATATAGTGTAACTTAT

ACTAGTTAATTAGTAACTATAGAGCCTTTTCCTCAGTTGTGTTTTGCTT

TTTTGGAGGGTGAAAATGTTATCACTATGAACAACAGCCAGGTTCCCAG

GCCAGAAGGACTAATTTTCTTTATAGTTATTGATTTTTAACTGTTATTG

ATTTGTAACTTATGAACTTTTAATGAGAGATCTACTGTGAGCTTCGAGC

CAAGGTTGTTAAATGATTTTATGTGCTTTTGCTGAATT

AAAGACACACGCATTATGGCATACGTCATCCCAATGCGTCACGCGCCAGAAGC

ATTGCGTTTTTAAAACAGGCTTTAGGGTTCGAACAGCATTGTTAAGGACTAAA

CAGTTCATTTTGTTATCCCAAACAATAAAAGAAAATGTTTTACTGAGGTTGTC

ATTCTGAACACATCCCTTTAAAACTGTTATCTCGCTTGAACACACATGCGTTT

ACTTTCATTCTGTTACTGTTTAAAGCAAAGAATCAATATGTTATGCTTAAGTT

AGTCTGTACTCACCACTTTGGAAACACTAAATCCGTATTATCCAAAGTTTTAG

TCCAGCGTTCAAACCAAGTGATGCCAAGCGCCGCTTCACTCCAGAACGTGTCA

GGTTTATCACGCGCCAGGCCAAACGCGTCTTCATATGTATGTTTTGATGAAAA

CCCACGAACAAAATGTGTCCTGTGCGTGCTGCAGTTATGGATTTGCTGCTCAC

ACGCACGCAAGAAAGTTGTCTTCACTTTTGGCAGGTAACACTGCCCGTACACA

CCTGTGCATGAAAACTTGAGACCC