ET 22-1: EGFP expression in the epiphysis and posterior tegmentum.

ET 22-2: EGFP expression in two symmetrical domains in the diencephalon ventral to the epiphysis, olfactory pits, hindbrain area juxtaposed to the ear and head epithelia.

ET22-1

Genomic location: Chromosome 8

The insertion is 4.4 kb upstream of dt1p1a10l (Pre-rRNA-processing protein) T SR2 homolog

DNA Sequence

ET22-2

Genomic location: Chromosome 5

The insertion is in intron of camkk1 (calcium/calmodulin-dependent protein kinase kinase1, alpha)

DNA Sequence

5’

5'

AGAGTTTGTATAATCATAAATATATGACTTTGTTCATCAATAACACTGT

TAAAAATATAAATCATGACTCTAACTTAAGCTTTATGTTTTAAAATGTT

TATTTATTGTTTATTTAGTAGTGTTTATTTTATGTTTATTTAATAGCAG

AGCCGGTTTGGTGCTGTTTCTCTTGGTTAATTGATTTCATCAAATACGT

TCACGATACAGACCACAATTTCATTTCATTACTGAAAGGCACATTTTAA

ACCAATTCTTCATGGATTTGCCCATTAAATGCCTGAATACAAAAAATGC

AAACATAAATCTAGTCAATATTAACTCTTTCCCTGCCAGCATTTTTGAT

TAGTTTCACAAAATTTTAATGGTGTTGACG

CTTACATGAAGCAAATTACATGAATATTTCATAGTAATCTACACTTGCCTATA

TCTAAAATTGTTTGACTTACAGGCTCTATGCACAGTAATACTATTTACTATTC

ACTATAATACTATTCCATTTAATATTTTTATTATCTACATTACTAGCCAATTA

GCCAAATTATAAGAGCTTTGCATATTACAAAAAAAAGATAAATATTTAATTTC

TTAAATATTTTATTTATTGTTTGCGATGCAAATTTAATTAAATCCACTTTATT

TGGTAAACAAAATAAGTCTCTCAAATAATATATCTACTAAATGACAGAAAATA

TTACTTTACAAACTGTATTGTAAATAAATCATAAAAACCTTTTCATATTAATC

AATAATACTACTGTAATTCATTTATAAACTGAATAAATATAAATTTTCACAAA

TAAATAACACAAGCAAAAATCATTGATGGGCTAAAAATCTTCGGAATTCTGTG

AAATTCTGCTCAAGCAgATTCGGTGTGGGCCTACCCATAACTCATTAAAAGAa

CAAGCACAACGCTGTTAGACCATTTTTCCATTCTTTAAAATAGCAAATTAAAA

TAGTATTC

3’

3'

CAAACTCTCGACTCACAGGTGACACCAAACTCAGAAATATTTCATTTCA

TATCATATAAATCACTCCATATTTCATCAAGGATATTTTCGTGACGTTT

TCCCCATCTAAAAGGGGATTTTAAACTTGATCAGTGTTTCTTGCTATAA

ATCGCCGCAGTTTTTTTCCCCTCATTTCAGGATCTCTGAGAAGTAAATA

ATGCGCCTCCACATGTATAACGCAGCAGCTTGGTTTGTTG

CATGTAAGGGCTTTATTGTTCTTCATTGTTGAGACCGTGACAGTATGAATTAT

TGAGGAATGAAAAGAATGAATAGCATGATGAATATTTGATAAGTAATGTAAGT

AGTATTACTCATTTTCATTTCATTCATGTTTTACACCCAATAGTGTATAGTGA

AATAGTGTAAGTTATTGACCATATCACAAAAGTATTTTGTGACCTATCGGTAT

CAACTAAAATTTTTGTTTCGCTTCATCAGAAATGTTTTAATAAATTCAAAAAG

ATCCATCAAAATAGTTTTGAATATATTTCTAGCTAAAGTACTGTCCTCTGCTC

TTGGTACTTGCAAAGTTTTGACCATCTGTTTGTGTGTGCAGGTGGAGGTTTTA

CTGCAGTGCAGGACTCACTGAGCATGTGCCAGGAGATCTAACTTTCCTCTCTC

TCTCTCTCTCTCTCaTCTCACTCTCTCTCTCTCTCT