EGFP expression in the olfactory pits, epiphysis, ventral tegmentum and anterior hindbrain, rhombomers 4—6 at the level of the ears, branchial arches, mouth, swim bladder, spinal cord and corpuscles of Stannius.

Insertion ET24-1

Genomic location: Chromosome 12

The insertion is 14.2 kb downstream of gfra1b (gdnf family receptor alpha 1b)

DNA sequence flanking Tol2 insertion site

Insertion ET24-2

Genomic location: Chromosome 11

The insertion is 113.4 kb upstream of LOC794258 (klhdc8a, kelch domain containing 8A-like)

DNA sequence flanking Tol2 insertion site

AGGAAAGCTCTTTTACTTCTGAAGCAATATGAATGTCATGTTCGTGTGA

CTCTTTGCGGTCTTTTGTTCCGCTTGGTTTTTCCCGTGGCTGGTTTCAA

GGCCTTTTTATTTGAACTTCTGAAATAATCAGCAGACACAACCCCTTTG

TGAAATTGTACGTCTCAAAATCCTGCTGACCCTGCAGAAGGCATGAATA

CTCCCTCTGAATTGTCACCCTCATGGCATTATTTCCCTTCTGATGTCGG

GTAATTTTTTCGGCTTGATTGCAAATTACACAGTACAGAGCTCATCTGG

TCACAAAGCTGATGCTTGTCTTTCGTTATTCAAGACCCCTTAAAAGAAA

GCAGGGCACATGGCACTGCAGAAACCAGACATTATGCCTAATTGCCTTT

GGTAATAACAAAGAGCCTCCTATCTTCGGCTTCCCCTCAGCTGTCTTAT

GAGTCACGTCCCTGTCC

CTCCTcttTCTGTCCCCCATGCaCAGTCATGTtaTGTCTTTCAGTCTCTGCTGA

TGAGCTGATGATCTGAATCAGGTGTGTTTGGTTAAGGAGACATGGGAAACGTGC

AAAGCTGGTGGTCCTCTAAGAACATGGTTGAGAAACACTGGTTTGAGTGATAGT

AGCAAGGCCATTACATATTAGGATATTAGTATATGACTTTCAAAAAAGGTAAAA

ACTACTTTTAAATTGTAGTATATCATGTCAAGAACCCAAAAAGTTGTGGTCTCT

TTATAAATATGGGCCTTATTCAATCAGTAAATGTTCTTGAAAATAGATCTATGA

AGTTACACGGACTACTTCTGTACCCATTATGACCTCGTATTCTAAATAGCAGAC

ATCTTATGAAATAAAAATGGAAATTTTATTTGATTTCTATCCAAAAAAGAAGAA

AAACTGATTTGAGA