EGFP expression in the olfactory pit, notochord, gut, skin and pericardium.

Genomic location: Chromosome 1

The insertion is 2.3 kb downstream of cnga3b (cyclic nucleotide gated channel, alpha 3b)

DNA sequence flanking Tol2 insertion site

5’

3'

GCTTTATGTGTTCACCCAGGCTTTTAAACGACAAAATTGAATGGCAGCT

ACAAAACAAACATAAAAAGGCTAATGAAGTGTACACAACTATACCCTGA

TTTCTATTTACATTTCACAGTTCAGTCTCTCTATGTTCTTCTCAAGAGT

TAAACCTGCGACGTTTGCATTTCCGRCACAGCTCCCTAGCTACCTTTAT

ATGTTTAGCAGGTTTAATTGCTGGGCTGATGCTCCCAATGGACAACCAA

CACCAAAATCAAAGATCGTCACATTGAGGGCAAGCCTGAACAGGAACAC

GGCCACAACGACTGCATGGTATGATTGGATAACTTGTAGACTTGTAGAG

TTTTCCACCCAACAAAACCAATCAATCCAAATTGAACATGAAATCATAA

GAATGTTTCTAAAATATATTTATTCAATACAGCAAATACATCCCTTGTT

TTCAGCAAGTATACAAATCAAATATGCATACAAGAAACGAACATGGTGA

ACTAAAATGGGAGATTCTCCATTCTATTCTCATCTCAAAAATAAACTAA

AACCTTCTAGTTCATTATATTAAGGCAAGCTCACACGATACACCTTTTA

GATTTTGCTGTCGCATGTAGGTTTGGGTCGATAGAAGATACCAAAGACG

GCATCGATGCTAAGACGGCATCGCCCCCATCTCAAACCCGCATGCGAAA

AATACACACTTAGGCCCCGTTTACACTAATACATCGTAGTTTTAAAATG

GCATTTTTAGAATGAAAGTGATCCAGATCCACACCCTTGTTTTACCTA

GCATTTCTGAACAGCCCTCCGTCTGCTGAAAACACGCATCACGTG

CATAAAGCCACACAGGTCATATTGATTCTGATTTTCTCTGTTTTCATGC

ATTTAATCAGAAATCTAATCAAGTATTTTTCCTCCCCAGGTCCAAAGTA

TGAAAGATGTTGATTACGAAAAGCGGCAGAGAGTTATCAGCATAGTTGT

AGCAACAGCAGCTTGTAATGCAAATGACATGACGTTTATCTGGCGTCAA

CCTACCGTGAACTTTGTGCAGTGCCGACTCTAATTAGACTGCGTTTTCT

CCCTTTTTTTCTCCAGAGACAGGAGTCAAGCAGCGTGCTATTCTGAGGT

AAATAACACGGAGGTGTGAGCAGTGCAACAAAACTGTTGAGGATGTGAA

ACTGTCAGACAGAATAGAGTCGCGCTGGTGTTTGTTTGAGTGAACAGCA

GACCGTGAAAATGGTGGCTTGTTAACTTCCTTCGCCTGTTTTTGAAGCC

TGCACTTTCTTACATTCGACGTCTGAATTTTATTTTGTCATGTTGA