EGFP expression in forebrain (neurons, ventricle, olfactory, habenula), midbrain (floor plate, roof plate), hindbrain (choroid plexus, floor plate, dorsal), spinal cord (floor plate, roof plate) and trunk pigment cells.

Genomic location: Chromosome 14

The insertion is in the intron of ccrn4la (CCR4 carbon catabolite repression 4-like a (S. cerevisiae))

DNA sequence flanking Tol2 insertion site flanking Tol2 insertion site

3:CTTTAAACTATATCATGTTTAGTTCCTACCCTGCTCCAACATACCTGTA

TGTTTAAAAAAAAAAGCCTAAAGGACTCAATTATTTAGATCAGGTGTGTTT

AATCAGGGTTAAAGCTAAACTCTGCAAAGCTGCCCCCCTCAAGGAACTGAG

TTTGGCACCCCTGCACTATATGGAAGGTAGCATCTGAAAATGCATAAGGCA

CTTTGACTTTGTCTATTTTAATCATTATAAAAAAATATAGCATGTTTCAAT

GCAAAATACACAGTAGTACATGAAAGGCGACACGCAAAACTTCTTCCAGAA

CAAACA