EGFP expression in the forebrain (olfactory), spinal cord (interneurons, ventral neurons), branchial arches, ear, jaws, eyes (lens) and notochord.

Genomic location: Chromosome 16

The insertion is in the intron of me1 (NADP-dependent malic enzyme)

DNA sequence flanking Tol2 insertion site

5:GAAATGAGGGAAACTCTGGGTTCAAGTCTTACATCAAGTCGATTTCTAA

AAGGGAGGTAAAATTCTCAGAGTGGGTTACCATGACAACTTATAATGAACC

ATGATAACATGATGAACCTAACCTGGTCAGAAGCATATTTTCCAACCCAGG

CTTATTTTGAAAACGTACCCCCACTTTTTTTCTGGAGAGAGCAAAATACTT

CCCAGGAGCTAAGTTTTTTTTTTTTTTTT

3:CTCATTTCAGGGAAACCAGTTCAGAGTTTTTACTTAACCTCTTAGCAAA

AGAATGACCTGAATTTGAAGATCAGTTTTTCCATTACTTTTCCATTTCTCC

CACTATAATCCAACTTAAAATCAAAAATCCTTTCCTCATTTAGTATTGTCA

TATAGGAGTGTATAAGATTGTATTGAGTGTGTATGTGTTTGTACACATATT

GATGGTTTTATCATTGAGCCTTCAGCAGAATGTTGTCTGTAAACAGACTGA

TCTCTGTGGAAATTATCAAGCAGGGAAGTCTGAATCTGAGCCATGCTTCTG

GTACCATAGTTTGCTTACCACTAAAAAGTCCACAATTCATAAAAGGAAGTG

CTGGTGTGTTGCCAGACAGTGTATTATTGA