EGFP expression in the dorsal midbrain, hindbrain (in rhombic lips at the ear level and hindbrain-spinal cord boundary), ventral spinal cord, eyes (lens) and notochord.

Genomic location: Chromosome 23

The insertion is in the intron of ENSDARG00000029146 (LRP1, low density lipoprotein receptor-related protein 1)

DNA sequence flanking Tol2 insertion site

5:GATGTAAATGAGAAATGTTGGCGCTGCTCATGAGTGCCCTTAATTTGCA

CTCTTCAGTCATTTGTCGTGTGGAAATAGACATGACCAGGTTGTACATACG

AGATGCAGAATCAGACACTCAAGACTTACGCATGCAGATTTATTGTAAGGG

GAAGAAAGACCTATTTGTGACTTGTGTAAGAATATTTTGACGATAAAACAT

CTTTAAATAGAATGTGTATTTTTAAATGATATTAGAGAACATTTTTTATTC

AGGAAATATTT

3:TTTACATCCTTTATTATCTCTGCCAGCAGCAAACTGGCACAACATCTGC

TCCTCTCCATATGGAGCTTACTAAACAAGCTGAGAGGAAAAGAGGCTAACA

TGTCAAAAAAAAAAAGCTAGGGCTAACCCAAAACTATCAAATACGCAGGGG

TATATACTTGTACGGACCCCGGGAGGGGATGCA