EGFP expression in the forebrain (olfactory, optic nerve), hindbrain (neurons), spinal cord (interneurons, ventral neurons), pronephros and ionocytes (and see Vasyliev et al., 2009, PLoS Biol.7(1): e9).

Genomic location: Chromosome 22

The insertion is in the intron of ENSDARG00000027618 (Grip2, glutamate receptor interacting protein 2 )

DNA sequence flanking Tol2 insertion site

3:CTTTAAACTATATCATGTTTAGTTCCTACCCTGCTCCAACATACCTGTA

TGTTTAAAAAAAAAAGCCTAAAGGACTCAATTATTTAGATCAGGTGTGTTT

AATCAGGGTTAAAGCTAAACTCTGCAAAGCTGCCCCCCTCAAGGAACTGAG

TTTGGCACCCCTGCACTATATGGAAGGTAGCATCTGAAAATGCATAAGGCA

CTTTGACTTTGTCTATTTTAATCATTATAAAAAAATATAGCATGTTTCAAT

GCAAAATACACAGTAGTACATGAAAGGCGACACGCAAAACTTCTTCCAGAA

CAAACA