EGFP expression in the forebrain (ventricle, epiphysis, habenula, olfactory), midbrain (neurons), hindbrain (choroid plexus, dorsal rhombic lips at the ear level), spinal cord (ventral neurons, roof plate), pronephros, eyes (lens), swimbladder and neural crest (see also Garcia-Lecea et al., 2008, PLoS ONE 3(9): e3090).

Genomic location: Chromosome 24

The insertion is 4.2 kb upstream of csrnp1b

DNA sequence flanking Tol2 insertion site

3:CTGTAGAGGTTCTGCAGGATGAGGCAGATTAGGGGAATTCTTAGTTCTG

ATTCAGAGTCTGAAATATCTGATGCTGATTATCTAGATTCTAGACTACGTG

TCACAGGGTCAATCTGGAATTGCTGGACTGAGTAGACAACTAGGGTATTAC

AGGAGCAAGCATTACCCTACTCAATGTAGAGCAAGAAGCCACAAGGCACAT

GCAATGGTACAATATACTGGATGTCACCACCCTAAACTTTGCCCAAACACC

CTAATTACATAGGTGGTGTGACCCATGTATGTAAACTTGATGTTGTAATAC

AAACCTAAGAAAAAATAAATAATTCATTTGCGCTACATGAAAAAAATAATT

TTTGAAAATTGATGAATAATTAAATTCATGGAATAATTAATATATAATGCT

GAAATAAATTGCTTTCAAAAGATAAAGCCAAAAA