EGFP expression in the midbrain (neurons), a gland in anterior intestine and eyes.

Genomic location: Chromosome 18

The insertion is 1.5 kb downstream of ppp1r13l (protein phosphatase 1, regulatory (inhibitor) subunit 13 like)

DNA sequence flanking Tol2 insertion site

5:ACTATACTTAACATTACTCATAACATCACTATATCTTTCCCATTGCAAT

GATTCTGATTGGTCCTCTTTGTGCATTCAGTGGGCTAAAATAAAAGCCTAT

ACAACCAAGTTAACTGCTCTTTTTCTAGTTTCACTGTCTAAACTGGTCTCC

TTTCTTCAGTCTCATTTTCAATCTTCTGTTAAACACAGTTCCCAGTACTGG

GTTGCAGCTGGAAGGGCATCCACTGTGTAAAACATATGCTGGATAAGTAGG

CGGTTCATTCTGCTGTGGCAACCCCTGATTGTTAAAAAAAAACTAAGCTGA

AGGAAAATGAATGAACACAGAAGAATGTTTGAAACCAATAACCATTACTTT

TCAAGTATTTGTTTTTCCTAAAATGGTCACATTTGGAAAGGTTACAGGTTT

CCAACATTCTTCAAAACATCTTTAAAAATATCTTTTAAAAGCCTTATATTG

TGCTAAAAAAAAAAA

3:AGTATAGTTCTCTGTTTTAAATTCATGCCACTAGAATTCCAGTTAAAAT

AAAGAATTTTTACATGAGAAAGTGACCAAATTACAGTTGTACAACAGCTGT

CTAAACCAAAAGGAAATATAATGTGTTCATTTACCAACTATGAGTTAGCAT

TAATCGTAACTTTGCCATATGTCAATACTGTTACTGTTGATGGAATCCAAA

AAAAACTTTAAAGCTGAATATCTCAAAACTCAGGACTTTTTTAGATAAGAA

ATGTTCTTCTTTTTATAAAAATAGACAAAATTAGGTTTGTCTTCACTGCTC

TAgtactattgtcattaaaccgtggaaacccctttgcaaatttgtccAAAC

TCTTCAGCTTGACCCTAGTCCTTCTGATTGAGTTCCCACACCGTACTCCCA

GCTTGGTGCTGGATACTAGTTCTGGTCAGCATATTGCATACCTGTATTTTC

TGTATGTAAGATTGCGCTAG