EGFP expression in the forebrain (neurons + donor), midbrain (donor), hindbrain (donor) and spinal cord (donor).

Genomic location: Chr. 14

The insertion is 5.3 kb downstream of ENSDARG00000077166 (Coiled-coil domain-containing protein 69)

DNA sequence flanking Tol2 insertion site

5:CTATTAGATATAGACTTTCTAACGTTATGAAAGTTATAACGAGAGAGCG

CCCATTGGCTATACAGTACACATGTAATTCTACTAGTCGCCACTAGAGAGC

TCTGTGTCGCAGTCATGCGCGCTCCTCCCTCGTTGGCTTCATTCCCGCGGT

TCAAATCAGATAAGATACAACATGTTTGCAGTGACTACTCGTCTAATTTGT

CGGTATGATAGCGCCATGTGTCTGGTTAGTTTAAAAAAAATGACGGCTAGG

GCTGGATAATGAAATAACAGCACCCTTTATCGTCaGTTAAAAGAACAGTAG

GCCTAAATTAAACTCTAAAGACTTTATTTACACTAAATACGACCTATTTTG

GAGGACTTGCTGATGCAATGTAAGTGTAATAGGCTAATTCTGCATAAATTA

TTAACGTCTACTTTGTTTGTTTGTGCTATTGTTTG

3:TCTAATAGCATGGCATATTTATATTTATATTTCATAACAACATATCTCTG