EGFP expression in the roof plate, cranial neural crest and later on meninges of the brain (see also figures in Parinov et al., 2004).

Insertion ET33

Genomic location: Chromosome 14

The insertion is in the exon of zic6 (zic family member 6)

DNA sequences flanking Tol2 insertion site

5’

3'

TACATGGGAAGCAACTCACGACTGTCATCCGCCAATGCTTTTTACACGA

CCGAATTACAGACATTACAAATCACTGTTACAGTGTTTGGGTGAAAAAG

TGTCCACGGCGCTATGGCAGGTGTACCAGCCATTTACCAGACTTTTTTG

GAAAGGTGAGTTTGGCGCAAAAGTTCTATGGCTGGGTGCGAACGTATGG

CTTGGTTGGTTGCATGAGTACTGAGCTGACTCGGGTCTGTAACTGCCCC

GTTGTAGCAACAGAGGGTCCAAAAGGGACTGTGGCCTGTGCGCCGTATA

GTCCAACGTGTTTTCAAAGGTGTGATGGAAACGTGACCTCACAGCAGAC

TCGTTTCGCGTCTCGGGAGACAGAGTCTCGTGAGAGGGGGTCGTCATCG

TTCGCGTCACTGTGGTTGAAGCGGGTGTGTCTTGATGTTCTGTCACCTC

AGGAGACCTGGCCTCTACAAGGTGCTCGTCGTCCTCGCCGATTTTGGCA

ATGTAGGCCTTGCAATGGAGTTTCATGTGTTTGCGCAAGGAGCTGGGAT

GTGTGTAACATTTTTCACAGCCTCTGACCTTGCACGTGTAGGGTTTATC

GCTGGAGTGAACGTGAGAATGCTTCTTCCGGTCACTGCTGTTTGCGAAT

CTTCGATTGCAGCCCTCAAACTCACATTTAAACGGTTTCTCACCTAAAC

GGGCAGAGGAGGGGGAAACGCGAGTTACGGGTGGGATGTACAGTCAACC

ATGGCTCATCAGTTCTGTGCATGGTGTCATTAGATGTGTTATGCAGGAT

GTTGTACCAAAAAAGTGTGCCGCTCCACACATTAAACAGTAAAGCAGGC

GAGAACATCACTTTGTTCAGATAACACTCATGTGCATGCAAAATGTGAT

AATTTTCTTTT

CCCATGTAAAACTGTCATAAAGGCTTTTCTGAGTAGAATAGAGCAATGTGA

TATATACATGTGTGTCTGTGTGTGTGTATGTATGCATATATACACACACAAT

ACATTCATTCAGTGAGACGTCGGTTATTATTTCTGAAACTGTAAATAGTTTC

CATAACCTTGCAAAAATAGTAGTTGACAAAATATAAATTATATATCAAATAT

AAAACAAATCTACAAATAAGACCCGGCAGATGAAGTAAAAAATATATAAGTA

TTATAAATAGATCGAAATAATTATATTATTTCTGCACAAGAAAACAAAAATT

ACTGGCGTATTATTTTGAGTTTCCGTAAAGGCAGGGCCTCCGTTTCTGATGT

AAAGTAGAAAAAGAGGTTTATCTCTTTAAAATTAATTAATTAAGGTAATAAT

ACGTGAAATCTTTTGTAAAGTACAACTTTTACATGTGTTTTCTGTCACAGCA

TAAATTTAGGTCGAGGGTTCTTATAATTAAGGTTCTCGTTTGTCTTGATGTG

TGTATGTGAAACGAACCCATCAGTGTACGAGGCCTTTAGCTACAAATTATTT

TAAGATTTTCTCATCACATTACAATTAAGTTTATAAGTTGATAGG