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ET 37

EGFP expression in the lens, retina, skin, fins (mesenchymal cells of fin folds), skeleton and anus (see also figures in Parinov et al., 2004; Zhang et al., 2010, Nature 466: 234-238)

 



 

Genomic location: Chromosome 20

The insertion is in the intron of scfd2 (sec 1 family domain containing 2)

 

DNA sequences flanking Tol2 insertion site

 

5’

3'

 

GAAATATTCCACTGAAATTTTGGTCCCTATTGACATGATAGCATCACGA

TTTGTCGGCTGCACATCCATGATGCGAATCTCCTGTTTCACCACGTCCT

AAAGGTGTTCTAATGGATTGAGCTCTTGGGACTGTGGAGGCCCTTTGAG

TACAGTGAACTCATTGTCATGTTCAAACCAGTCTGACCATTCTCCTCTG

ACCTCTGGCATCAACAAGGCATTTGCCGCTCACTGGATATTTTCTCTTT

TTTAGACCATTTTCTCTTTTTGTGAATGAAAATCCCAGTATGTCGGCAG

TTTCTGAAATACTCAGACCAGCTTGGTCYAGCACTATCAACCATGCCAC

GTTCAAAGTCACTTAAATCTCCTTTCTTGTCCGTTCTGATGCTTGATTT

GAACTGCAGAAGATCGCCTTGACTATGTCATGCCTTAAGACATTAAGTT

GCTGCCATGTAATTGGCTGATTAGAAATTTGCATTAACAAGCAGTTGGA

CAGCTTTACCTAATAAAGTGGCCGGTAAGTGTGTATTTTGCAAACATTT

GCCATTATGCACTTCCCGACTTAATAAATGATTTCACAGAAATGCCTGA

CATTATACACTACACAACTGAGGATTATATACTATCAGACTTTCAGGTC

TTTCCAAGCACAACAAACTTGCCTAGAAACATGTGGCTTGCTGATAGAA

GATGCAGGACAGATGAAGCAAATGTGGAAATATACTGTGCAGGAGATGA

GCACAGTAAACAAGCACAATAAACAAGCCGCTGATCACCACCCAACTTG

CTGTTGTGATTGTTGTCCATGTAATGACAGCGTTTGGTTCT

 

AATATTTCCAGAACTTTGTTGAATCTATGCCACGAAGCATTAAGGCAGT

TCTGAAGGCAAAAGGGGGTCCAACCCGGTGCTAGAAAGGTGTACCTAAT

AAAGTGGCCGGTGAGTGAATGTTTGCAAAGTGTTATAAAGTAATCTCTT

TTCTTGAGATGATAATATTGTTCTTATTCTAAACATTTCTGATAATACT

GTATTTTTTATATATAAACTGTACATTAAATGACCTGTTGTTGTTTTGT

TATTTAAATTATACTGAAAATATGTGATTCATTAATTTTCAATCAATCT

AGTTCAAAATTAAACAGGCAAACTATAACTTATGTAAATTAACAACTCT

AATACTTTTTTTTTATTTGAATGCTTTTAAACCTATTTTAATTTTTGAA

CCACATAGCAAGGAAAATGTGAGTGTTTTTTAATCTGAAGTATATTGTC

TTTCTTCTATCATTGAAATAATCACTGCAAAATTGAAAATGTATTGAGA

AGACAAAAACATGCTACACAAAACCCACCAAACACAGATAGGGATCCAA

AGTTGTCAGATTTTGTTCTAGGATAACATGAAAAAACCTGAGAATGTTA

TTTCTGAGTTCATAGACTCACGCACTGAGAACTTAAGTATGAAACTTAA

GTTGTCAAACTGCTGACCTACTTCAAGTCCTCTCAGCAGAACGCATCAG

TAAAATGAGCGTACGCGTTTAATCAGACATGATGTACTAATTAGCCAAT

GAGGGCAGTGAAGTGAGTGTGTGTAATGTGATCTGAGGCTGAATCCTCT

GGCTCGGCCCTCAGCATTATGTTCAAGAGTGAAACCTGAACTGCCCTGG

AG