EGFP expression in many different organs and tissues, including the lens, olfactory pits, epiphysis, mouth cavity (taste buds), optic tectum, midbrain-hindbrain boundary, branchial arches, fins, spinal cord, pronephros, yolk cell and hindgut.

Genomic location: Chr.23, 39022377-39022786 (5' flanking sequence).

This insertion is in the intergenic region 23kb upstream of sall4 and transgene expression pattern recapitulates that of this gene.

DNA sequence flanking Tol2 insertion site

5’

3'

GGGCAGATACTGTTCATAACACAACAATGCATGAGTTTGTGTTGCCAGATCACT

GTTCTTAACACAACAATGCATGAGTTTGTGTTGCCAGATCACTGTTCTTAACAC

ACCAATGCATGAGTTTGTGTTGCCAGATCACTGTTCTTAACACACCAATGCATG

AGTTTGTGTTGCCAGATCACTGTTCATAACACAACAATGCAAGAATTTGTGTTG

CCAGATCACTGTTCATAACACAACAATGCATGAGTTTGTGTTGCCAGATCACTG

TTCTTAACACACCAATGCAAGAATTTGTGTTGCCAGATCACTGTTCATAACACA

ACAATGCATGAGTTTGTGTTGCCAGATCACTGTTCATAACACAACAATGCATGA

GTTTGTGTTGCCAGATCACTGTTCATAACACAACAATGCATGAGT