EGFP expression in the midbrain (floor plate, neurons), hindbrain (floor pate, neurons), spinal cord (floor plate, interneurons), ionocytes, branchial arches and notochord.

Genomic location: Chromosome 14

The insertion is 2.4 kb upstream of foxa (forkhead box A sequence)

DNA sequences flanking Tol2 insertion site

5:AGTTCGAGGCTAATACATCTAAAATAAAGCTACTTTGATGCATGGTGGAG

CCAAAATATCAAACGGTGACTGTGATTCCACCTCATTAAACATCTGAAAGAC

ACATTTCCATCACTTTTGTCAATGTGTGAACAGCTTAGCCTAATAAAATAAA

TAAATAAACGTCAATAGGCTATCTATTAAAGCCTGCATATTATTTTTGTTGC

GGTTTGATGCTGTTGAAAATTGCGTGTAGTACTTGTTCTGATTTTGTTGGTA

CTTAATACTGGTATCAGTATTTGCCTCAATTATTGAAAAGTAACCTCACTTA

TCTTTTCTGTCAGTCTTTTGACTGTTGATTTGGGCTATTTTATATACAATAA

CCATGTGTGAAGCAGTTCACTGTTCTAAAGTACTAATAACTGGATTTGTGCT

GTGACGACTCTCTTTCTTCAGCTCTTCTTGTT