EGFP expression in the forebrain (epiphysis, habenula), midbrain (MHB, interpeduncular nuclei), hindbrain (neurons), ear, fins, eyes.

Insertion 1

Genomic location: Chromosome 13

The insertion is in the exon of hif1an (Hypoxia-inducible factor 1 alpha inhibitor)

DNA sequences of Tol2 insertion site

Insertion 2

Genomic location: Not Determined

DNA sequences of Tol2 insertion site

5:AGGAAAACACAGCCAGTGTTTCTGTTTTCACGCTGCTTGTATGACTGCGC

GGGTCGCTCGTCTGTTCGTGCCTCGGCGGGGTGTGTGAAGCCCAGGAGCGGT

TCTCGAGCTCGCCGGCTCCCCTTTCAGACCATCGGCCTCTCGAGGTTTCTTT

GTGCTGCCCCAGTGATCCTCTGGCGGACTCGGAGGACATGGCGGAGACCGAC

GGAGCCGCCGCCTTCACGGAGCTGCGCGACCCGGACTGGGATGAGTCGCAGC

TTCGACAGTATACTTTTCCAACCCGACAAATACCGCGACTGTCCCATACGGA

TCCGCGTGCCGAGGGGCTCATCAATAACGAGGTAAGCTAGCATATTTATCCT

GCATTATTAACGCTGCAAGAGTGAGCGACCGACGGAGGAGAGGAGAACAGCA

AACGTCTGTTTGTGGGTGCAAGGTGCTTTAAAATTAAAAAATGATTTACCCA

AACTCAAGCAGCAGGACAGTATTAAACGCTTTGTTTGGATACAAAATGCTGA

AATTAATGCCAAAACTATTTGATGAGTTGGGTCATTACGTCAGATAAAAAA

5:TTTCCCACACTTTTACCAGAAGAGGTCCTCATCGAGCTGATTTAGAAAGC

TTCGAGTAACGAACCTTTTTCCGATACAATTGAGTCGAGCGCTTCACTGGTT

CAGAAAGCTTCATTTCACCATCACTACTGCGGGGAGGTACTGTGATTCAAAG

CGACACCCCCTGGCCTAAAAAATCGCTAGCGCGTCACAACAGCACAAACACC

TATC

3:GTTTTCCTACTCATAACACCCACCGGAAGTAGGCGTTTCAAAATAAAAGT

CATTCTATAGTTTCTTGCTGCCTGGTGTTATTCGTTATTCGTGAATGAATGG

CTGCTGAGCAATAAGGAAAGAATTACAAACATGTCAATCGTTCCAAAAATGC

AGCACAAACAACTGAA

3:GTGGGAAAGCACTGTGTTGCCATAATGTCAAATGTTCACAACTGACCAAC

TCATTCATGTAGTAATAAAAAAACAGCAATATAAACAAATTACTCAATTACT

GTAGTTTTTACACAAGATATATTACATTACACAACTGATGATTGTAGTAAAA

TCCAAGGTATTCAAAAGTTTATCATAATACAATTAGTCCCGCTCCAAGCAGG

GATCGAACCAGCGGTTTCTGGTTGGGAGGCGAGTGCTCTAGGAGGACGCTAT

GTGAACGATGCTTCAGGGTTGCATTCACCAAGGGCGTATTCCAGAAAGCAGG

TTATGTGACATACCCGGGTAAGTTTGAGAGTAAGTAAGTGGATAACCTCAGC

TTTCGGTTTCAAAAACGAAGGTAACTTTGTGGGTATGTATGTAACCATAGCA

ACTCACTCTCTGAAGATAACCTGCTCGGTAGCAGGTTATGTTCCAGTGTAAG

TTTGTGT