EGFP expression in the forebrain (neurons), midbrain (neurons), hindbrain (neurons), spinal cord (neurons), eyes, ear, neural crest, fins and branchial arches.

Genomic location: Chromosome 24

The insertion is 50.3 kb upstream of ENSDARG00000075542 (similar to Zinc-finger homeodomain protein 4)

DNA sequences of Tol2 insertion site

5:GATACTAGTATCCATTGATAAGTACTTGGTATACAATAAGTACTAAATAA

CCAATAAATTTCTTTGAGTACATAAAAAGTATTTATTAGTCAGAGTTGAAAT

ACTTACTAGCCAAAAACAGAAGCCCAAATCTCATATTTGGTACTAATAACTA

ATGAAATAAATTCTAGAATCTAATGAATATTTATTAGGACCCATTTAATAGG

TACAAGTATCTACCAAATAAGTATCCAATGAATGAGTACTAATATCTAAAGC

AGTGGTCTCAAATCAATTCCTGGAGGGCCACAGCTCTGCATAGTTTAGCCCC

AACCA