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GATEWAYS 2A---GATEWAYS 84B  
  Gateways 35

EGFP expression in the hindbrain (posterior neurons, floor plate), spinal cord ( roof plate (anterior), anterior floor plate, branchial arches, sclerotome (anterior) and PNS(?).

 


 

Genomic location: Chromosome 19

The insertion is in the exon of hoxa5a (Homeobox protein Hox-A5a)

 

DNA sequences of Tol2 insertion site
 

 

 
 

5:GGAACGGTTCTCAGCCGGGAGGGAGTTTCGAAAGTGTCAAGCCCTATGGA

GGAAGAAAAGCCACCGGGTAGGGGACAGACTGCTTCACAGAATGTGTCGGAG

GCACCGCAAATTTATCCATGGATGCGAAAACTTCATATAAGTCATGGTAAAC

GGCAAACGTAGAGGTTTGCTTTTATTGCGTTTGTGTTCTGAGTGTTAATGTA

AGGTGTGATTGGCCTCCTGCCAGTTTTACAGTAAAAATTTTTTTAACTTTAT

ATGAATATTGAAATATTAGAGTTAGTTTTTCATTTTGGATATTTATATCGAA

AAATATGCCAAATATGCATCCTGGGGTCTAATGACTCCATGTTTCTAAAGAT

TATTTAAAACTGATTATATTCTTTAAAAACGAAGTGCAATTGTATGATCAAT

TAATTTGTCGATTGTGGATTAATAAATTGGTGGTGTTATTAAATAAATTATA

ATTTGTTAGAG