EGFP expression in the forebrain (habenula, ventricle), midbrain (meninges, neural crest, roof plate), hindbrain (meninges,neural crest), spinal cord (roof plate (most anterior) ), eyes, notochord, neural crest and heart.

Genomic location: Chromosome 2

The insertion is in the intron of ENSDARG00000057284 (similar to vertebrate pim oncogene family)

DNA sequences of Tol2 insertion site

5:GTTGATGACCTGTTGATATGTGCTTAAATGAAAGATGTGTGTGAAAGACA

CTGTGACTCTCCTTAAGCATTTGGCTAAGGAGGGCCACAAGGTTAGTTTGAC

AAAATTGCAGTTTGGTAAACAAAAGGTAACTTTTTTGGGGCACGTCATTACA

CCACACAGCAAATCTCTGTCTGAAAAAAGGGTGAGTGGTATAAAAAATGTAC

CAAAACCACAAAAAAACAAAAAAAAACAAATGTTGTCTTTTTTGGGTATGTG

CTCATATTGTCCCACATTTATTCCAAATTATGCAATTTTGGAACAACCCCTG

AAAGCCCTAACACTAGGGAAGGGGATGAAATCCACTGACAAACTAGGGGGGA

CAACAAAGGCAAAGCAGGCATTTGTAAACATGAAATTACAAATGGCTGAACC

CCCTGCATTGGGTTTACCTGTACCAACAAAACCATTTGTTCAAATGGTAAAT

CAAAGGAGTGGGTTCATGACGTCTTTGCTCCTACAGAACCATGAAGGTAGAC

TGTTAAACAATGTACTGGTTTGAATGCA

3:TCATCAACATACTGTAACAAAGCAGTTCCAGCTGTCAGGGTCAAAGGTTC

CAAACCTCTTAACAACGCTTCATTTTATAAAGTGAAACAGAACCTGACACAG

ACATGTAAAGGTGTTGCCTTTGCCATTGAAATTAAATGCAAACCAAAACTGG

CTGTCTTTGTCCACTGGCAGACTGAAAAATGCATTAGCCAGATCAACCACTG

AATAGAATTGTGATTTTTCAGGAATTTGTGA