EGFP expression in the midbrain (neurons), spinal cord (neurons), eyes, lens, ionocytes, neuromasts and corpuscles of Stannius.

Genomic location: Chromosome 17

The insertion is 66.9 kb upstream of prox1 (prospero-related homeobox)

DNA sequences of Tol2 insertion site

5:GTGCGTGAGGAGGGAGGACGTCTTGCTCATGCTTCTATAATCGATTCTAA

AAACAAATATCGAAGCAAATTTGACAAAAAATGCCAAAAGGTTGTGGAATGA

GCCACTTTATTATGTAATATTGTGACTAAACTCTGCCTCCGCAAAAAAAAAT

CAATGGTTCTAAATCGCAGCCTGTTGAGCTCCACCCACTGATTTAGTAGGAA

AATAATGAGATGGGATTATTATCAGAAAACAGTGGTTGATTTTTATTTATTT

TTTTATATATATATTTGAATTTTTCAATGAATCCATACACATAACTGTACTT

TTAAACCTTTAGGGGCAGATAAAGTCATTAAAACCAACACAGGCTCATTGTA

AAAACGATCCCCTGGATACATTTCTGGAGAATGCAAATTATGTAGCCAGAGC

TATGTATGACTGCATTTTGTCTTTAAAA

3:TCACGCACTCATATTGCACTTTATGTTCCAGGCTGAAGCACACTTACATC

ATTATGATGCGAGAGAAGCTGTTAAGCGATGACCCCTACATGCCCACCGAGC

ACAAGCCAAACTAAGTCTAGCTGTGCCCCAGCTCTTCCACGGACAGCTGACG

AGTCAGTCTTAGGGGGTCAAACACGCTTCATAGGCACGATTCAGATCATCAA

GTGTGAGTACATCCTTACAGCACAA