EGFP expression in the forebrain (neurons), ventral gland (head), gut, cleitrum, notochord and pronephros.

Insertion 1

Genomic location: Chromosome 1

The insertion is in the intron of ENSDARG00000069471 (similar to major histocompatibility complex, class I-related)

DNA sequences of Tol2 insertion site

Insertion 2

Genomic location: Chromosome 8

The insertion is 31.3 kb upstream of ENSDARG00000058731 (similar to solute carrier family 2 (Facilitated glucose transporter), member 6 )

DNA sequences of Tol2 insertion site

5:AAAACAAGCCCACAAAAAGGCGGGACTGGGCAAGGCGGGTAATCGGG

AGGTTATGATATCTGCATTTACTTTCTACCTTGTCCCAAAGCTGAAGGC

AAATATTTCCAAA

5:ATGTAAAGTCAATGGGGACCCCTTTGGGACGTCCTAGCACCCCAGGGGTA

CAACTTATACCCCCTTTGGGGGTATGTTCTCATACAGGCTGCAACCCCCTAC

AGATGTTGCGAATCCCATATTTCTACAAATTTCACACTTGGCGCTATGACGC

TTTAAAGGTCGTCCCATTTCGCGGGGGCGTTAGTGCTGTTCTAGAAAAATGA

ATGGGAGTCAATGGGGCCCATGTTAGTCAATGGGAGCACTTTGGGACGTCCT

AAAGCCCCAGGGGTGCAACTTTTACCCCCTTGCGAGGTATGTTCTTACTTAG

GCTGCAACCCCCTACAGATGCTGTGAATCCCATATTTCT

3:CTTGTTTTGCATTGTCAGTGAACGTTTTTCTGTTTTTTTTTATATTA

TTTCCAAGAACCAGCATGTACAGTTTTTTACAACAAAATCACTTTTCAT

TTCTTAACAAGAAATGAATGAATGAGAAAA