EGFP expression in the forebrain (epiphysis, olfactory), midbrain (floor plate, neurons), hindbrain (floor plate, neurons), spinal cord (floor plate), notochord, horizontal myoseptum, ear, branchial arches, lens and yolk.

Genomic location: Chromosome 24

The insertion is in the intron of lama1 (Laminin subunit alpha-1 precursor (Laminin A chain)

DNA sequence flanking Tol2 insertion site

5:CCAACTCATTCAACATATTTTTTTACACAGCAGATGGCCTTCAAGCTGCA

ACCCAGTACTGGAAAACACCCATACACTCTCATTCTCACACACACACACACA

CACACacacacacactATGGACAATTTTATCTTATGGAGCACCAAAAGGAAA

CCCACGCAAACGTGGGGAGAACATGCAAACTCCACACAAAAACACCAACTGA

CCCAGTCGGGACTCAAACCAGCGACCTTCTTGCTGTGAGGCGATTATGTGGG

ATTTTTTAAAAACAAACTAAAATATTTAAAAATATAATCTTTTCAGTATGTC

TTTTTATTTAATATCATTTAATGCACTAAAATTATTAAAAACTAGGGCGGGG

TGTTATATACACATATAAAAACATGAATACTCAAGTACATTGCGAAAATACA

AATTTGTTCACTTGTTTTGGATGCATTTGTGATTGTGATTAATCACGGCCCA

GGATTAATAAAAACTAAAAATAAATGACATAAAATATTTCATATATTAAAAA

TATAAATTAATATATTTTTTAAATTCATCATATTTTTATTTAACAAATAAAT

AAATTAAATATATTTTAATCACAACAAACTAAAATTATTTTAAAAAATACAA

AAAAAAAATGTAGTGTACAGCTAATTTTCTAAAAC

3:TGAGTTGGTGGTTCATACCACTGTGGCGACCTCTGATTAATAAAGGGACT

AAGCCAACGATAAAATAAATGAATGAACAATTTGAGATAAGTATACTACTTT

TGGGTGGCACAGTGGTTAGCTATATGGCCTCACAGCACTCAAGTCTCTGCTC

CTCCAC