EGFP expression in the forebrain (floor plate), midbrain (floor plate), hindbrain (floor plate), spinal cord (roof plate), lens, lateral line and ionocytes.

Genomic location: Chromosome 7

The insertion is 6.9 kb downstream of efnb3 (ephrin B3)

DNA sequence flanking Tol2 insertion site

5:ATGAAGTCGCAACATCGGTGCCCCGCTAACGTGAGTAATAGTGTGTTAT

AGTAACGTAGTAGTGTTGGAGGACACATGTTATTATCGATTGCGCTTCATA

ACAGGGACAATGACGGATGTATGATTATGTATACAAAAACCAGGTGGTTGT

TCAACTTTGCCAGTTAATGACGTCATCTGCACGCCGAAATGTTTGGACTTA

ATGTTAATGATTCTGCAAATACAGCCTAGGCTTAAATAACTTTATATTACG

ATTTGTATTTTGTAAATTTATTACCACTTCATATTTCCAAGTGTGGAAATT

CATCTTTGACACTTTCATAAACTCAATCAAGCAACATTCCCTTTACTGTGC

ATTCATAACGAAACACATACAAGATAGTAGCCTACACACACGTACCCCCTT

GCATGTTGTAGTTTTTAAAGATAATGATTAATTTAACCCTCTATTATTTAA

TAAATCTTTCAATAACCTCTTAAAATGTGTTTTTCTTAAAAAAAAGAGTCT

ATATTAGGCTACTTGCCATTTTATGCCTAGTTAATACCAGTCTGGACTTCA

TTTTGGCTTCAAAAGTGCCCTAATTCTTTGTGGCATAGATTTAGGAAGGTG

CTAAAAACATTTCACAGAGATTTT

3:GACTTCATGATGAAATTCTCGAATTCTGACTCAAAGCGCGTAGCAGCGA

AATCATTTGCATGACCCGAACATGAAGGGCGCCCGGAAGTTCACGGAGCTC

GTCGCATAAGCGGAAGTTACCGACAAATTATGAGATCTTTTTTAAAATGTG

TTTGCTCTTGAACTTGTTCTCGAGTATGGAAAATATTGGGCAGAAAAACAA

TTTGTTTATACCAATGCTAATCTCT