EGFP expression in the forebrain (olfactory, ventricle), midbrain (neurons), hindbrain (neurons, choroid plexus), spinal cord (roof plate), pronephros, horizontal myoseptum and PNS (trunk).

Genomic location: Chromosome 24

The insertion is 121.7 kb downstream of zgc:153639 (solute carrier family 22, member 13)

DNA sequence flanking Tol2 insertion site

5:ATTATTCCAGTTATATATACCAGACCTGAAAAGCATCT

3:GGAATAATTAACTCCAATCCATTAAATTATCTGATAATCCATAGTCTT

TTTTTTGCATTTACA