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BAC transgenic zebrafish for transcriptional promoter and enhancer studies.

TitleBAC transgenic zebrafish for transcriptional promoter and enhancer studies.
Publication TypeJournal Article
Year of Publication2015
AuthorsKraus, P, Winata, CL, Lufkin, T
JournalMethods Mol Biol
Volume1227
Pagination245-58
Date Published2015
ISSN1940-6029
Abstract

With the advent of BAC recombineering techniques, transcriptional promoter and enhancer isolation studies have become much more feasible in zebrafish than in mouse given the easy access to large numbers of fertilized zebrafish eggs and offspring in general, the easy to follow ex-utero development of zebrafish, an overall less skill demand and a more cost-effective technique. Here we provide guidelines for the generation of BAC recombineering-based transgenic zebrafish for DNA transcriptional promoter and enhancer identification studies as well as protocols for their analysis, which have been successfully applied in our laboratories many times. BAC recombineering in zebrafish allows for economical functional genomics studies, for example by integrating developmental biology with comparative genomics approaches to validate potential enhancer elements of vertebrate transcription factors.

DOI10.1007/978-1-4939-1652-8_12
Alternate JournalMethods Mol. Biol.
Citation Key25
PubMed ID25239750