memKR expression in the retina, lens and pigmented epithelium, olfactory placode, whole heart (Teh et al., 2010), telencephalon (olfactory bulb), hindbrain (cerebellum, r3, r5-6, radial cells), jaws, enveloping layer, notochord.

Genomic location: Chromosome 19

The insertion is 48bp upstream of exon1 of ENSDARG00000036090 , pard6gb (mutagenic). The mutant phenotype is similar to that of the ENU mutant (Munson et al., 2008, Dev Biol, 324, 41) with fragmentation of the central canal in the neural tube.

DNA sequence flanking Tol2 insertion site

5':

3':CTAATCAGTACCTCTCACTAGGGGGAGTGGGCTAAGTTTTCACATATGACATCATCGCT

CTGTCTCCGACGGGTTGCTAATTCTACTACGGTGAGGGTTTGGCTTATGAATATTAAGTACG

TAACGATGGTCTTCCTTAGAGGTAGCTGATTGGCTGCAAAGCGAACGTTAGTAAGCGGGATA

AGGTGATGCGGTTGATTTCTTGATTAAAGGGACAGTCACATTTTTACATGCATAAACAAAGA

TGCATGGCTTCAATCCTAAATTTTCGACATTTTCAGATTGAAGTTGACGCTCAGCTCAAATC

TTACAACAAAACAACAAAAATGTGTCCGAGTTTTGGTACTATATGAAGAACTGTCGCTGTTG

ATTCTGATTTGAGTAAGATAGCTTTTCTGTAATGCTCTTAATTTATTTATTTTCATACACTT

TTGTTAAATTGTGATTTACTTACCCCCTTCTTTGTTTTGTTAGTCTTTCTCTCTCTG