memKR expression is found in the choroid plexus III and IV, branchial arches, olfactory pits, rhpmbic lips (r4-5), vagal nucleus, pectoral fins, otic vesicle, EVL.

Genomic location: Chromosome 8

The insertion is in the 5'-untranslated region of ENSDARG00000013607 , sema3gb and probably recapitulates expression of this gene. The expression pattern of this transgenic line is very similar to that in ET33-E20 transgenics(Gateways, Garcia-Lecea et al., 2008; Korzh et al., 2011) as illustrated by overlap of two signals in the choroid plexus (image 3).

DNA sequence flanking Tol2 insertion site

5':GGCACCTCACTGTACAGTAGGTGACATGACAATGTAAACAAAGAACT

CCGAGATGAGCAGATAAACAAACCGTATCAGTCATTCAAACAAGCCTTAT

ACTAAATGCTTAAATCGTGTTTGCTTTTTGAAAAGAGCCACTTACTTTTT

GTCCAGGTAACTTAAGGTGGAAGATGTTACATTCCGTGGCACACAACTGT

CCGGTACGAACAAAAACGGTCACCAAAGGCACATACACGCGCCAATGTCC

ATCTGTTCGTTCTTTCTCAGTTACCAAAGTTTCCAGTCGCCGCAGTTTAT

ACAAAAGTACAGGCTAGCGTTCATAAGCCGGGTACTCTTTGAGAGAAAGA

AGCTACGCAAAGCTCAAACAAACTTTCCCATTTCCAATCGCACTGTCATC

CTTCACCATATCACGAGGGGAAAAATCCGTTAATATTCCAAAGTCAAACG

CGCGCTCCCTCTCTGTACAAGTCCAGTCAAGTGTTTGCAGGTGTCTAGTG

TGCTGTGTGCTGTACCTCGCCCTCTCTCCACTACCTCCCTATTAAAACCA

GCCAGCCGTGTCAGTGTTAACCAGCTCCCTGCCAGCGCACATGCAACTGC

GCGGAGCCCC

3':